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Literature summary extracted from

  • Xu, Y.; Li, Y.; Li, L.; Zhang, L.; Ding, Z.; Shi, G.
    Reductase-catalyzed tetrahydrobiopterin regeneration alleviates the anti-competitive inhibition of tyrosine hydroxylation by 7,8-dihydrobiopterin (2021), Catal. Sci. Technol., 11, 3128-3140 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.5.1.34 additional information the purified recombinant enzyme from Escherichia coli is used for 5,6,7,8-tetrahydropteridine (BH4) regeneration to alleviate 7,8-dihydropteridine (BH2) inhibition of L-tyrosine hydroxylation by crude tyrosine hydroxylase (SrTH) from Streptosporangium roseum DSM 43021 as part of a combined whole-cell catalyst, and a series of tyrosine hydroxylase/sepiapterin reductase (TH/SPR) synthesis systems, overview Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.34 gene nfsB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.34 quinoid 7,8-(6H)-dihydropterin + NADH + H+ Escherichia coli non-enzymatic reaction of quinoid 7,8-(6H)-dihydropterine to 7,8-dihydropterin 5,6,7,8-tetrahydropterin + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Escherichia coli P38489
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 quinoid 7,8-(6H)-dihydropterin + NADH + H+ non-enzymatic reaction of quinoid 7,8-(6H)-dihydropterine to 7,8-dihydropterin Escherichia coli 5,6,7,8-tetrahydropterin + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.34 7,8-dihydrobiopterin reductase
-
 Escherichia coli
1.5.1.34 DHPR
-
 Escherichia coli
1.5.1.34 EcDHPR
-
 Escherichia coli
1.5.1.34 NfsB
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.34 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.34 7.4
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 NADH
-
 Escherichia coli